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Isolation of a developmentally‐regulated expressed sequence tag from bladder tissue using the mRNA differential display
Author(s) -
Chaqour Brahim,
Howard Pamela S.,
Macarak Edward J.
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201643
Subject(s) - biology , complementary dna , differential display , northern blot , gene , in situ hybridization , genbank , microbiology and biotechnology , messenger rna , gene expression , fetus , homology (biology) , cdna library , suppression subtractive hybridization , sequence analysis , expressed sequence tag , genetics , pregnancy
In order to gain insight into the molecular and cellular events that govern the structural and the functional properties in developing organs, we have conducted a study to identify genes that have a temporally‐restricted expression in the bladder wall during fetal development. We utilized the mRNA differential display technique and compared the pattern of gene expression during the first, the second and the third trimester of gestation. We cloned and sequenced a cDNA fragment (bld‐10) which was expressed during the second and third trimester but consistently absent during the first trimester. The bld‐10 sequence is not related to any known gene in the GenBank database but has significant homology (89%) with human expressed sequence tag (EST) that has been cloned from human fetal heart and brain libraries. When used in Northern‐blot hybridization as a probe, the fragment bld‐10 generates two hybridization signals of 3.1 and 4.0 kb, that are minimally expressed during the first trimester of gestation and up‐regulated in the second and third trimester. Differential expression of this gene may be responsible for some of the profound changes which occur during organ development.