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Effects of glucose on the activity of erythrocyte membrane Ca2 ATPase in subjects with normal and impaired glucose tolerance
Author(s) -
Bilgin Ramazan,
Tükel S. Seyhan
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201601
Subject(s) - medicine , endocrinology , in vivo , atpase , tunicamycin , in vitro , incubation , chemistry , calcium atpase , biology , biochemistry , enzyme , endoplasmic reticulum , microbiology and biotechnology , unfolded protein response
The effects of glucose on Ca2+ATPase activity of erythrocyte membranes were investigated under in vitro and in vivo conditions. Results of the study, in vitro, showed that Ca2+ATPase activity of erythrocyte membrane was decreased by increasing glucose concentrations (0‐30 mM). When glucose‐6‐phosphate (0‐100 mM) was used instead of glucose no significant change occurred in Ca2+ATPase activity. Tunicamycin (0.01 ng/ml) when added to the incubation media together with glucose prevented the decrease in Ca2+ATPase activity. The results of the studies in vivo showed that Ca2+ATPase activities of both male and female subjects with normal or impaired glucose tolerance were changed with time in a complex manner after an oral glucose loading. However, Ca2+ATPase activities of the subjects with impaired glucose tolerance were smaller than those of the corresponding normal controls even before glucose administration.