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Transblot identification of avidin‐interacting proteins in rat salivary glands
Author(s) -
Shirakawa Satoko,
Murata Takatoshi,
Mochizuki Hajime,
Takehara Tadamichi,
Kobayashi Shigeru,
Haneji Tatsuji
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201542
Subject(s) - avidin , peroxidase , submandibular gland , blot , chemistry , staining , collodion , microbiology and biotechnology , parotid gland , salivary gland , conjugate , biochemistry , membrane , biology , biotin , pathology , enzyme , medicine , endocrinology , mathematical analysis , mathematics , gene
Blotting of rat parotid gland proteins separated by SDS‐PAGE and transferred to Immobilon transfer membranes revealed that avidin‐peroxidase conjugate interacted with bands having estimated molecular weights of 72, 74, and 120 kDa. Even at the lowest concentration of avidin‐peroxidase used in the general ABC method (1:2000 dilution), three bands were clearly discernible. The staining reaction of parotid gland proteins was eliminated on preincubating the proteins with native avidin. The staining reaction was markedly reduced with the proteins obtained from submandibular/sublingual glands.