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Isolation and characterization of Saccharomyces cerevisiae SAB2, a suppressor gene for temperature‐sensitive phenotype of ars‐binding factor 1 mutant
Author(s) -
Shin YongHyun,
Goo DuckMo,
So InSeop,
Rhode Peter R.,
Campbell Judith L.,
Kim Jiyoung
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201533
Subject(s) - biology , mutant , gene , microbiology and biotechnology , saccharomyces cerevisiae , plasmid , genetics
A high‐copy number suppressor of yeast abf1‐5 mutant, a temperature‐sensitive lethal mutant, was isolated and named SAB2 (suppressor of ABF1). Hybridization to a yeast chromoblot and to prime clone grid filters revealed that the SAB2 gene was located near the yeast SUP3 on chromosome XV. The suppressor activity was contained in a 2.5 Kbp DNA region of the SAB2 plasmid. The nucleotide sequence of the DNA region contained a long open reading frame, which turned out to encode for yeast tryptophan permease. Four putative ABF1 binding sites were found in the promoter and the structural regions of the tryptophan permease gene. Binding of ABF1 to two of the sites tested in this study was detected. Our results indicate that ABF1 may be involved in the transcriptional control of the yeast tryptophan permease gene.