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Expression of cecropin CMIV fusion protein in E. coli under T7 promoter
Author(s) -
Xie Wei,
Qiu Qifeng,
Wu Haihong,
Xu Xianxiu
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201531
Subject(s) - fusion protein , microbiology and biotechnology , cecropin , plasmid , recombinant dna , fusion gene , expression vector , signal peptide , gene , dna , peptide , chemistry , biology , biochemistry , antimicrobial peptides
A synthetic gene coding for cecropin CMIV from Bombyx mori was cloned into plasmid pEZZ318 and fused to a DNA segment encoding the signal peptide of Staphylococcal protein A and IgG binding domain. The fusion gene was then subcloned into expression vector pET11c under the control of T7 promoter and expressed in E.coli. The fusion protein did not exhibit any antibacterial activity either in cell lysate or in medium. After cleavage from the fusion protein with CNBr the biological activity of recombinant cecropin CMIV was obtained.