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Effect of cycloheximide on the expression of LPS‐inducible iNOS, IFN‐β, and IRF‐1 genes in J774 macrophages
Author(s) -
Hattori Yoshiyuki,
Akimoto Kazumi,
Matsumura Michiko,
Tseng ChenChiang,
Kasai Kikuo,
Shimoda ShinIchi
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201503
Subject(s) - cycloheximide , gene expression , microbiology and biotechnology , irf1 , gene , protein biosynthesis , cell culture , biology , interferon gamma , regulation of gene expression , interferon , chemistry , lipopolysaccharide , protein synthesis inhibitor , cytokine , immunology , biochemistry , genetics
The effect of cycloheximide (CHX) on the gene expression for inducible NO synthase (iNOS), interferon (IFN)‐β, and IFN regulatory factor (IRF)‐1 was examined in LPS‐stimulated J774 macrophages. LPS caused increased expression of mRNAs specific for iNOS, IFN‐β, and IRF‐1 with different kinetics. Addition of CHX resulted in inhibition of the LPS‐induced iNOS gene expression and parallel decrease in NO production. In contrast, expression of IFN‐β and IRF‐1 genes in response to LPS was potentiated in the presence of CHX. These results indicate that de novo protein synthesis is not required for IFN‐β and IRF‐1 gene expression and that ongoing protein synthesis including IFN‐β and IRF‐1 may be involved in the induction process of iNOS in mouse macrophages.