Premium
Nucleic acid‐8‐methoxypsoralen crosslinks bind monoclonal anti‐Z‐DNA antibody
Author(s) -
Arif Zarina,
Ali Rashid
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201483
Subject(s) - nucleic acid , dna , monoclonal antibody , chemistry , epitope , denaturation (fissile materials) , antibody , microbiology and biotechnology , biochemistry , nucleic acid denaturation , biology , immunology , base sequence , nuclear chemistry
Abstract Native calf thymus DNA and poly(dA‐dT) poly(dA‐dT) were photo‐adducted with 8‐methoxypsoralen and characterized by thermal denaturation (Tm) and hydroxyapatite column chromatography. The data demonstrated the formation of interstrand photo‐crosslinks. It has been shown by competition ELISA and band shift assays that crosslinked species of DNA‐8‐MOP and poly(dA‐dT)‐8‐MOP photoadducts recognize previously defined monoclonal anti‐Z‐DNA antibody (Z22). The results indicate the possible presence of Z‐ or Z‐like epitopes on nucleic acid‐8‐MOP crosslinks as Z22 antibody does not recognize other nucleic acid conformations. These studies also point out that conformational changes in DNA arising from the photo‐addition could induce antibodies to DNA or could cause autoimmune disease.