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A novel method for purification of catalytic antibodies toward DNA from sera of patients with lymphoproliferative diseases
Author(s) -
Kozyr A. V.
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201441
Subject(s) - polyclonal antibodies , dna , affinity chromatography , oligonucleotide , antibody , chemistry , ion chromatography , enzyme , microbiology and biotechnology , chromatography , sepharose , ion exchange , biochemistry , biology , ion , immunology , organic chemistry
A novel highly efficient method of purification of DNA‐hydrolyzing antibodies from sera of patients with lymphoproliferative diseases was developed. The method suggests purification of serum sample using Cibacron Blue 3GA, ion exchange chromatography on DEAE‐Sepharose FF, PrG affinity chromatography and selection of the DNA‐binding fraction on DNA‐Sephacryl. The application of the method allows to obtain the stable and active polyclonal preparation with increased content of DNA‐hydrolyzing antibodies characterized by specific binding constant determined using heteropolymeric oligonucleotide substrate. The unusual properties of anti‐DNA catalytic antibodies in respect to their interactions with DEAE ion exchanger are discussed.