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Gingko biloba extract EGb 761 is a suppresser of AP‐1 transcription factor stimulated by phorbol 12‐myristate 13‐acetate
Author(s) -
Mizuno Masashi,
DroyLefaix MarieTherese,
Packer Lester
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201431
Subject(s) - jurkat cells , signal transduction , transcription factor , transcription (linguistics) , phorbol , chemistry , messenger rna , microbiology and biotechnology , dna , second messenger system , protein kinase c , biochemistry , biology , gene , t cell , genetics , linguistics , immune system , philosophy
Transcription factors are activated in response to a wide variety of extracellular stimuli. The present study investigated which step Gingko biloba extract (EGb 761), a flavonoid phytochemical rich preparation can affect signal transduction during AP‐1 activation. Kinetic experiments with human Jurkat T‐cell lines demonstrate that it takes 1 to 1.5 h to activate AP‐1 DNA binding activity. These data indicate that c‐fos mRNA is expressed and then AP‐1 DNA binding activity is activated. Pretreatment of Jurkat T cells with 10 µg/ml EGb 761 suppresses AP‐1 DNA activation and c‐fos mRNA expression. These results suggest that the step in the signal transduction pathway for AP‐1 activation that is inhibited by EGb 761 is upstream to c‐fos mRNA expression.