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Sle autoantibodies recognize spermine induced Z‐conformation of native calf thymus DNA
Author(s) -
Ali Asif
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201393
Subject(s) - spermine , dna , antibody , chemistry , microbiology and biotechnology , autoantibody , biochemistry , biology , immunology , enzyme
The binding specificity of naturally occurring anti‐DNA antibodies with spermine modified DNA was analyzed by direct binding and competition ELISA. The binding was visualised by band shift assay. The SLE anti‐DNA antibodies appreciably recognized the spermine induced Z‐conformation of native DNA. The degree of binding of these antibodies to the Z‐conformer was almost similar to their binding with native DNA. The spermine‐ DNA complex was found to be thermodynamically more stable than native DNA.