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ATP‐induced calcium mobilization in glomerular mesangial cells is mediated by P2U purinoceptor
Author(s) -
Takeda Michio,
Kawamura Tetsuya,
Kobayashi Mami,
Endou Hitoshi
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201382
Subject(s) - adenosine , adenosine triphosphate , desensitization (medicine) , calcium , chemistry , endocrinology , microbiology and biotechnology , medicine , biology , biochemistry , receptor
To identify the functional P2 purinoceptor subtype in glomerular mesangial cells (GMC), polymerase chain reaction coupled with reverse transcription (RT‐PCR) were performed, and cytosolic free calcium concentration ([Ca2+]i) was determined. RT‐PCR analysis revealed that the molecular identity of P2 purinoceptor localized to GMC was both P2U and P2Y. The rank order of potency in stimulating [Ca2+]i was ATP≒UTP>2‐methylthio‐ATP>ADP≒adenosine≒AMP. In addition, cross‐desensitization between ATP and UTP occurred. In conclusion, ATP induces increase in [Ca2+]i via P2U purinoceptor in GMC.