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Drosophila melanogaster genomic DNA sequence homologous to mammalian cytosine DNA‐methyltransferase gene
Author(s) -
Vanyushin Boris F.,
Poirier Lionel A.
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201381
Subject(s) - dna methyltransferase , dna methylation , biology , genetics , genomic dna , gene , dna , microbiology and biotechnology , cpg site , southern blot , illumina methylation assay , gene expression
Using Southern blotting procedure we have shown that Drosophila genomic DNA hybridizes with 4423‐bp C‐terminal fragment of murine cytosine DNA‐methyltransferase gene. Thus, the Drosophila genome has a sequence homologous to the mammalian cytosine DNA‐methyltrasferase gene. We assume that DNA methylation most likely responsible for strong CpG suppression in the Drosophila genome mainly was catalyzed by a cytosine DNA‐methyltransferase that has since been lost.