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Structural analysis of two plant 5S rRNA species and fragments thereof by lead‐induced hydrolysis
Author(s) -
Ciesiołka Jerzy,
Krzyzosiak Włodzimierz J.
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201341
Subject(s) - ribosomal rna , rna , protein tertiary structure , protein secondary structure , 5s ribosomal rna , nucleotide , chemistry , hydrolysis , nucleic acid structure , biology , 18s ribosomal rna , crystallography , stereochemistry , biochemistry , gene
The structure of plant 5S rRNA species from lupin and wheat germ as well as the structure of two RNA fragments that represent domains β and γ of lupin 5S rRNA have been probed by Pb(II)‐induced hydrolysis. The lead digestion patterns of 5S rRNA species show that the secondary and tertiary structures of the molecules are very similar. The data suggests that two potential base pairs at the bottom of helix E are destabilized and this causes an enlargement of the hairpin loop e. On the other hand, nucleotides from loop c seem to be involved in the formation of some kind of higher order structure. A comparison of the distribution of cleavages induced in RNA fragments to those in the corresponding regions of the entire 5S rRNA shows that under conditions applied in our studies the structural domains β and γ are not involved in formation of any tertiary interaction within 5S rRNA structure.