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Stem cell factor protects bone marrow‐derived cultured mast cells (BMCMC) from cytocidal effect of nitric oxide secrected by fibroblasts in murine BMCMC‐fibroblasts coculture
Author(s) -
Park SungJoo,
Jun ChangDuk,
Choi ByungMin,
Lee EunJung,
Kim HyungRyong,
Cho HaeWon,
Chung HunTaeg
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201323
Subject(s) - fibroblast , 3t3 cells , cell culture , mast cell , microbiology and biotechnology , stem cell factor , biology , bone marrow , cell growth , stem cell , chemistry , immunology , haematopoiesis , biochemistry , transfection , genetics
The survival of mast cells are dependent on two kinds of growth factors, one derived from T cells (IL‐3) and another derived from fibroblasts (stem cell factor [SCF]). The 3T3 fibroblast cell line derived from WCB6Fl‐+/+ mouse embryos (+/+ 3T3 fibroblasts) supported the proliferation of bone marrow‐derived cultured mast cells (BMCMC) in the PWM‐stimulated spleen cell conditioned medium (PWM‐SCM), whereas the 3T3 fibroblast cell line from WCB6Fl‐Sl/Sld mouse embryos (Sl/Sld 3T3 fibroblasts) did not. To study the role of nitric oxide (NO) on the growth of mast cells in BMCMC‐fibroblasts coculture, we used a NO synthase inhibitor, NG‐monomethyl‐L‐arginine (NGMMA). NGMMA recovered survival and maintained proliferation of mast cells in BMCMC‐Sl/Sld 3T3 fibroblasts coculture. Sl/Sld 3T3 fibroblasts as well as 3T3 fibroblasts from NIH‐+/+, BALB‐+/+ or Swiss‐+/+ mouse embryos secreted NO in PWM‐SCM, but not in α‐MEM. SCF protected BMCMC from cytotoxicity of exogenous NO in IL‐3‐supplemented α‐MEM. We concluded that SCF might protect BMCMC from cytocidal effect of NO in BMCMC‐fibroblasts coculture.