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Fluorescence study on ligand induced conformational changes of human erythrocyte glucose transporter
Author(s) -
Xie W. S.,
Yue J. C.,
Tu Y. P.
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201291
Subject(s) - fluorescence , quenching (fluorescence) , glucose transporter , chemistry , transporter , conformational change , biophysics , nanosecond , photochemistry , biochemistry , biology , endocrinology , laser , physics , quantum mechanics , gene , insulin , optics
The nanosecond time‐resolved fluorescence and fluorescence lifetime quenching have been used to detect the conformational changes of human erythrocyte glucose transporter induced by its ligands. Results show that D‐glucose can decrease the fluorescence lifetimes, while L‐glucose exhibits no effect. The fluorescence lifetime quenching results also show that in the presence of D‐glucose, the quenching efficiency of hypocrellin B (a hydrophobic quencher obtained from a parasitic fungus, growing in Yunnan, China) is lower than in the presence of L‐glucose. It can be deduced that the conformational changes of human erythrocyte glucose transporter induced by D‐glucose are different from those induced by L‐glucose.