Premium
Immunochemical studies on human, bovine and pig brain regulatory subunits of cAMP‐dependent protein kinase type II
Author(s) -
Sveshnikova E. V.,
Alexandrova N. A.,
Grozdova I. D.,
Sveshnikov P. G.,
Severin E. S.
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201222
Subject(s) - monoclonal antibody , protein subunit , enzyme , antigen , microbiology and biotechnology , antibody , protein kinase a , chemistry , biology , biochemistry , gene , immunology
Regulatory subunits type II (RII) purified from human, pig and bovine brains were compared using 11 monoclonal antibodies (MoAb) against bovine brain RII. Bovine RII has at least 5 antigenic sites located in the N‐terminal 1‐110 residues. Immunochemical difference detected between human and animal RII was more pronounced than between pig and bovine RII. Certain MoAb influenced R‐cAMP binding and holoenzyme formation. RII of the three species responded to MoAb attachment in a similar fashion. The results suggest that anchoring of neural protein kinase via the N‐terminal part of RII may influence the enzyme activity.