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Zearalenone ‐ Induced uncoupling in plant mitochondria is sensitive to 6‐ketocholestanol
Author(s) -
Macri F.,
Vianello A.,
Braidot E.,
Petrussa E.,
Mokhova E. N.
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201152
Subject(s) - uncoupling protein , mitochondrion , uncoupling agents , chemistry , zearalenone , biology , biophysics , biochemistry , botany , mycotoxin , adipose tissue , brown adipose tissue
Zearalenone (F‐2) is a mycotoxin which acts as a protonophoric uncoupler in plant mitochondria [Macri, F. and Vianello, A., (1990) J. Plant Physiol. 136, 754‐757]. In the present paper, the mechanism of F‐2‐induced uncoupling in pea mitochondria was studied. The uncoupling by F‐2 was partially reversed by 6‐ketocholestanol (kCh) under conditions in which kCh completely reversed the FCCP‐induced uncoupling and almost did not affect the palmitate‐induced uncoupling. Recoupling effects of carboxyatractylate, ADP and cyclosporin A were small and could not essentially decrease the kCh‐insensitive part of F‐2‐induced uncoupling. It is suggested that a protein, mediating kCh‐sensitive uncoupling, is involved in the F‐2 effect in plant mitochondria.