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S‐(‐)‐nicotine‐1′‐N‐oxide reductase activity of rat liver aldehyde oxidase
Author(s) -
Sugihara Kazumi,
Kitamura Shigeyuki,
Tatsumi Kiyoshi
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201113
Subject(s) - nicotine , chemistry , enzyme , aldehyde reductase , aldehyde oxidase , reductase , aldehyde , cytosol , biochemistry , stereochemistry , medicine , xanthine oxidase , catalysis
In the present study, a rat liver cytosolic enzyme responsible for reduction of S‐(‐)‐nicotine‐1′‐N‐oxide to S‐(‐)‐nicotine was investigated. We found that aldehyde oxidase (EC 1.2.3.1) can function as a S‐(‐)‐nicotine‐1′‐N‐oxide reductase in the presence of its electron donor such as 2‐hydroxypyrimidine. The apparent Km and Vmax values of the enzyme for the N‐oxide were 0.24 mM and 30.3 nmol/10 min/mg protein, respectively.