Premium
Superoxide production by mitochondria in the presence of nitric oxide forms peroxynitrite
Author(s) -
Packer Michael A.,
Porteous Carolyn M.,
Murphy Michael P.
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201103
Subject(s) - peroxynitrite , superoxide , nitric oxide , chemistry , mitochondrion , hydrogen peroxide , oxidative phosphorylation , mitochondrial respiratory chain , oxidative stress , biochemistry , reactive oxygen species , biophysics , biology , enzyme , organic chemistry
The mitochondrial respiratory chain continually produces superoxide leading to high levels of mitochondrial oxidative stress. This oxidative damage has been attributed to the formation of hydroxyl radicals and hydrogen peroxide from superoxide. Alternatively, mitochondrial superoxide may react with nitric oxide forming the potent oxidant peroxynitrite, thus damaging mitochondrial protein, lipid and DNA. To test this hypothesis we induced mitochondrial superoxide formation in the presence of nitric oxide. Here we demonstrate that mitochondrial superoxide reacts with nitric oxide to form peroxynitrite, suggesting that mitochondria may be a significant intracellular source of peroxynitrite.