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Interactions of trans‐acting factors(s) with the estradiol response element and nuclear factor 1 of the vitellogenin II gene of Japanese quail
Author(s) -
Gupta Sanjay,
Upadhayay Rashmi,
Kanungo M. S.
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201032
Subject(s) - vitellogenin , quail , medicine , biology , gene , hormone response element , endocrinology , hormone , gene expression , estrogen , microbiology and biotechnology , chemistry , biochemistry , estrogen receptor , genetics , cancer , breast cancer
Abstract This study was directed at achieving an understanding of the mechanisms by which steroid hormones control the synthesis of vitellogenin (VTG) protein in the liver of the Japanese quail. Northern hybridization shows that administration of estradiol alone or with progesterone stimulates the synthesis of VTG mRNA. Gel mobility shift assay of DNA fragments containing the ERE and NF1 shows that estradiol alone or with progesterone increases the levels of nuclear proteins that bind to these cis‐acting elements of the promoter of VTG gene. The cooperative effect of the two hormones seen at the level of expression of the VTG gene may be due to protein‐protein interactions of trans‐acting factors that bind to ERE and NF 1.