Premium
Effect of IL‐1 in amniotic fluid on the fetal keratinocyte
Author(s) -
Kamada Masaharu,
Hirano Kohki,
Arase Seiji,
Daitoh Toshifumi,
Maegawa Masahiko,
Irahara Minoru,
Aono Toshihiro
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201011
Subject(s) - amniotic fluid , epithelium , keratinocyte , fetus , umbilical cord , andrology , immunohistochemistry , biology , chemistry , pathology , immunology , medicine , cell culture , pregnancy , genetics
IL‐1 α is predominantly increased in amniotic fluid of the patients with premature rupture of the membrane (PROM). In the present study we examined the effect of IL‐1 on the fetal keratinocytes, one of the main tissue exposed to the increased IL‐1. Exposure of IL‐1 at over 0.1 pg/ml IL‐1 significantly (p<0.01) inhibited proliferation of the keratinocytes in low calcium keratinocyte growth medium obtained from the umbilical cord. Immunohistochemical analysis using antibodies to various cytokeratins revealed that the umbilical cord keratinocytes is more similar to the mucosal epithelium than the skin epithelium. These results suggest that IL‐1 α increased in amniotic fluid in the PROM cases leads the immature proliferating mucosal epithelium to maturate for protection of the fetus against environment.