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Regulation of type II collagen expression by cyclin‐dependent kinase 6, cyclin D1, and p21 in articular chondrocytes
Author(s) -
Hwang SangGu,
Song SungMin,
Kim JeongRan,
Park ChunShik,
Song WooKeun,
Chun JangSoo
Publication year - 2007
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216540701245022
Subject(s) - cyclin dependent kinase , cyclin dependent kinase 6 , cyclin d1 , chemistry , type i collagen , cyclin d , microbiology and biotechnology , ectopic expression , cyclin d3 , type ii collagen , cyclin , cyclin a2 , cyclin dependent kinase 2 , kinase , cell cycle , cancer research , biology , endocrinology , protein kinase a , biochemistry , cell , immunology , gene , arthritis
This study examined whether cell cycle regulatory proteins, such as cyclin‐dependent kinases (CDKs), cyclins, and CDK inhibitors, regulate type II collagen expression and mediate interlukin‐1 (IL‐1β)‐induced suppression of type II collagen expression in articular chondrocytes. IL‐1β inhibited type II collagen expression, but activated CDK6. Ectopic expression of CDK2 did not alter type II collagen expression. However, overexpression of CDK6 inhibited type II collagen expression, whereas inhibition of CDK6 activity blocked IL‐1β‐induced suppression of type II collagen expression. IL‐1β upregulated the expression of cyclin D1, which is known to activate CDK6. In turn, overexpression of cyclin D1 suppressed type II collagen expression. In contrast to cyclin D1, IL‐1β triggered down‐regulation of the CDK inhibitor, p21. Overexpression of p21 blocked IL‐1β‐ or CDK6‐induced suppression of type II collagen expression. Our results collectively indicate that CDK6/cyclin D1/p21 complex regulates type II collagen expression in articular chondrocytes.IUBMB Life, 59: 90‐98, 2007