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Membrane Protein Solubilization: Recent Advances and Challenges in Solubilization of Serotonin1A Receptors
Author(s) -
Kalipatnapu Shanti,
Chattopadhyay Amitabha
Publication year - 2005
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216540500167237
Subject(s) - solubilization , membrane , membrane protein , chemistry , biochemistry , peripheral membrane protein , integral membrane protein , lipid bilayer , dissociation (chemistry) , biophysics , biology
Solubilization of integral membrane proteins is a process in which the proteins and lipids that are held together in native membranes are suitably dissociated in a buffered detergent solution. The controlled dissociation of the membrane results in formation of small protein and lipid clusters that remain dissolved in the aqueous solution. Effective solubilization and purification of membrane proteins, especially heterologously‐expressed proteins in mammalian cells in culture, in functionally active forms represent important steps in understanding structure‐function relationship of membrane proteins. In this review, critical factors determining functional solubilization of membrane proteins are highlighted with the solubilization of the serotonin1A receptor taken as a specific example.IUBMB Life, 57: 505‐512, 2005