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Evidence for Rat Organic Anion Transporter 3 Association with Caveolin‐1 in Rat Kidney
Author(s) -
Kwak JinOh,
Kim HyunWoo,
Song Joon Ho,
Kim MoonJae,
Park HyeonSeon,
Hyun DongKeun,
Kim Dong Soo,
Cha Seok Ho
Publication year - 2005
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216540500104750
Subject(s) - western blot , caveolae , gene isoform , kidney , organic anion transporter 1 , caveolin , caveolin 1 , transporter , extracellular , messenger rna , organic anion transporting polypeptide , organic cation transport proteins , biology , solute carrier family , blot , microbiology and biotechnology , chemistry , biochemistry , membrane , endocrinology , gene
The rat organic anion transporter 3 (rOAT3) has recently been identified as the third isoform of the OAT family. The mechanisms that regulate rOAT3's functions remain to be elucidated. rOAT3 contributes for moving a number of negatively charged organic compounds between cells and their extracellular milieu. Caveolin (Cav) also plays a role as a membrane transporter. To address the relationship of these two proteins, we investigated the protein‐protein interaction between rOAT3 and Cav‐1. The rOAT3 mRNA and protein expression were observed in the rat kidney, and the expressions of Cav‐1 mRNA and protein were also detected in the kidney. Confocal microscopy of the immuno‐cytochemistry experiments using primary cultured renal proximal tubular cells showed that rOAT3 and Cav‐1 were co‐localized at the plasma membrane. This finding was confirmed by Western blot analysis using isolated caveolae‐enriched membrane fractions from the rat kidney and immuno‐precipitation experimentation. When rOAT3's synthesized cRNA of rOAT3 along with the antisense oligo deoxynucleotide of Xenopus Cav‐1 were co‐injected into Xenopus oocytes, the [3H] estrone sulfate uptake was significantly decreased. These findings suggest that rOAT3 and caveolin‐1 share a cellular expression in the plasma membrane and Cav‐1 up‐regulates the organic anionic compound uptake via rOAT3 under normal physiological conditions.IUBMB Life, 57: 109‐117, 2005