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A Novel snoRNA can Direct Site‐specific 2'‐O‐ribose Methylation of snRNAs in Oryza Sativa
Author(s) -
Li Wei,
Jiang Ge,
Huang Bing,
Jin Youxin
Publication year - 2005
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216540500090819
Subject(s) - small nucleolar rna , biology , methylation , small nuclear rna , pseudouridine , rna , long non coding rna , gene , transfer rna , genetics , non coding rna
Small nucleolar RNAs (snoRNAs) are a kind of noncoding RNAs, and the vast majority of snoRNAs are involved in site‐specific modifications of rRNAs. A novel box C/D snoRNA called snoR124 was found in Oryza sativa, and it can direct 2'‐O‐ribose methylation of spliceosomal small nuclear RNAs (snRNAs). The snoRNA has two antisense elements, and the results of primer extensions at different dNTP concentrations provide evidence that snoR124 guide 2'‐O‐methylations of the C76 residue in the U4 snRNA and the T91 residue in the U5 snRNA. In addition, this snoRNA is located in a snoRNA gene cluster with another 7 snoRNAs which are identified to direct ribose methylations in rRNAs. This is consistent with the opinion that the snoRNA gene organization in plant is mainly gene cluster. The snoR124 is the first example of a snoRNA that directs modifications of RNAs other than rRNAs in plant; it will avail to get more insights into the function of snoRNAs in plant.IUBMB Life, 57: 173‐179, 2005