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Hydrogen Peroxide‐induced Cell Death in Arabidopsis: Transcriptional and Mutant Analysis Reveals a Role of an Oxoglutarate‐dependent Dioxygenase Gene in the Cell Death Process
Author(s) -
Gechev Tsanko,
Minkov Ivan,
Hille Jacques
Publication year - 2005
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216540500090793
Subject(s) - programmed cell death , arabidopsis , biology , microarray analysis techniques , catalase , mutant , microbiology and biotechnology , gene , heterologous , transcriptional regulation , gene knockout , transcription factor , gene expression , biochemistry , enzyme , apoptosis
Hydrogen peroxide is a major regulator of plant programmed cell death (PCD) but little is known about the downstream genes from the H2O2‐signaling network that mediate the cell death. To address this question, a novel system for studying H2O2‐induced programmed cell death in Arabidopsis thaliana was used. The catalase inhibitor aminotriazole (AT) reduced the catalase activity and caused endogenous accumulation of hydrogen peroxide that eventually triggered cell death. Microarray analysis with a DNA chip representing 21500 genes and subsequent comparison with other PCD‐related expression studies revealed a set of new H2O2‐responsive genes that were highly regulated in a common fashion during different types of PCD. These included an oxoglutarate‐dependent dioxygenase and various oxidoreductases, the transcription factors Zat11, WRKY75 and NAM, proteasomal components, a heterologous group of genes with diverse functions, and genes encoding proteins with unknown functions. Knockout lines of the oxoglutarate‐dependent dioxygenase exhibited significantly reduced death symptoms and chlorophyll loss upon H2O2‐induced cell death, indicating a role for this gene in the cell death network.IUBMB Life, 57: 181‐188, 2005