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Src Kinase‐Dependent Epidermal Growth Factor Receptor Transactivation in PPARγ Ligand‐Induced Suppression of Porphyromonas Gingivalis Interference with Salivary Mucin Synthesis
Author(s) -
Slomiany Bronislaw L.,
Slomiany Amalia
Publication year - 2004
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216540410001690456
Subject(s) - wortmannin , ciglitazone , transactivation , chemistry , proto oncogene tyrosine protein kinase src , mapk/erk pathway , cancer research , protein kinase b , microbiology and biotechnology , kinase , signal transduction , biology , peroxisome proliferator activated receptor , receptor , biochemistry , transcription factor , gene
Peroxisome proliferator‐activated receptor γ (PPARγ) has emerged recently as an important participant in the resolution of inflammation by conveying signals that lead to mitogen‐activated protein kinase (MAPK) cascade activation. In this study, we report that PPARγ activation leading to the impedance of P. gingivalis lipopolysaccharide (LPS) inhibitory effect on salivary mucin synthesis requires epidermal growth factor receptor (EGFR) participation. We show that activation of PPARγ with a specific agonist, ciglitazone, prevents the LPS‐induced reduction in mucin synthesis, and the effect is reflected in a marked decrease in apoptosis, caspase‐3 activity and NO generation. The impedance by ciglitazone of the LPS‐induced reduction in mucin synthesis was countered (up to 68.9%) in a dose‐dependent fashion by a specific inhibitor of EGFR kinase, PD153035, as well as wortmannin, an inhibitor of phosphatidylinositol 3‐kinase (PI3K). Moreover, the inhibitory effect of ciglitazone on the LPS‐induced reduction in mucin synthesis and upregulation in apoptosis, caspase‐3 activity, and NO generation was blunted by a selective inhibitor of tyrosine kinase Src, PP2, responsible for ligand‐independent EGFR transactivation. These findings indicate that PPARγ activation leading to the suppression of P. gingivalis LPS inhibition of salivary mucin synthesis involves Src kinase‐dependent EGFR transactivation.IUBMB Life, 56: 153‐159, 2004