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ACYP1 Gene Possesses Two Alternative Splicing Forms That Induce Apoptosis
Author(s) -
Degl'Innocenti Donatella,
Marzocchini Riccardo,
Malentacchi Francesca,
Ramazzotti Matteo,
Raugei Giovanni,
Ramponi Giampietro
Publication year - 2004
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216540310001654349
Subject(s) - exon , alternative splicing , rna splicing , hela , gene , amplicon , biology , microbiology and biotechnology , cell culture , ectopic expression , genetics , polymerase chain reaction , rna
In human cell lines two products of the ACYP1 gene were detected by RT ‐ PCR. In addition to the expected amplicon corresponding to the CT form of acylphosphatase (320 bp) a second unexpected one (400 bp) was characterized as the result of an alternative splicing in which an extra 79 bp long exon is inserted between the two known exons. This new product, indicated as CTsv, was cloned and expressed. We performed the ectopic expression of the two alternative splicing forms. Both CT and CTsv products were able to induce a proapoptotic effect when expressed in HeLa cell line, despite the fact that the CTsv protein did not show any acylphosphatase activity.IUBMB Life, 56: 29‐33, 2004