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Direct Visualization by Confocal Fluorescent Microscopy of the Permeation of Myristoylated Peptides Through the Cell Membrane
Author(s) -
EnseñatWaser Roberto,
Martin Franz,
Barahona Fernando,
Vazquez Jesús,
Soria Bernat,
Reig Juan A.
Publication year - 2002
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216540213823
Subject(s) - myristoylation , confocal microscopy , fluorescence , fluorescence microscope , biophysics , confocal , membrane , chemistry , peptide , cell , microbiology and biotechnology , biochemistry , biology , physics , geometry , mathematics , quantum mechanics
To study the permeability through the cellular membrane of synthetic peptides containing an hydrofobic moiety, we used a 13‐mer myristoylated peptide labeled with a N ‐terminal fluorescent probe. After 2 h of incubation, the subcellular distribution was analyzed in intact chromaffin cells by confocal fluorescent microscopy. Our results demonstrate that myristoylated peptides diffuse into intact cells, showing an heterogeneous distribution, but they do not reach the cellular nucleous, at least during the time range used.