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Synergistic Effect of Vitamin D Derivatives and Retinoids on C2C12 Skeletal Muscle Cells
Author(s) -
Stio Maria,
Celli Alessandra,
Treves Cristina
Publication year - 2002
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216540212334
Subject(s) - c2c12 , retinoic acid , incubation , thymidine , cell growth , cell , biochemistry , tretinoin , vitamin , myocyte , chemistry , medicine , endocrinology , biology , microbiology and biotechnology , myogenesis , in vitro , gene
The response of C 2 C 12 myoblasts to 1 nM 1,25‐dihydroxyvitamin D 3 [1,25(OH) 2 D 3 ], two vitamin D analogues (KH 1060 and EB 1089, which are 20‐epi‐22‐oxa and 22,24‐diene‐analogues, respectively), 100 nM retinoids (9‐ cis retinoic acid, all‐ trans retinoic acid) and to combination treatments, after 72 h incubation, was studied. The incubation with 1,25(OH) 2 D 3 was ineffective on either cell proliferation or [ 3 H]thymidine incorporation (expressed as DPM per cell) or protein content per cell. On the contrary, all the other treatments inhibited cell proliferation, this inhibition being synergistic when the vitamin D derivatives were combined with 9‐ cis or all‐ trans retinoic acid, and increased [ 3 H]thymidine incorporation and protein content per cell. The levels of the VDR protein remarkably increased in comparison with control cells, except for the incubation with 9‐ cis retinoic acid. This increase was particularly accentuated in C 2 C 12 cells treated with KH 1060 and 9‐ cis retinoic acid in combination. These results, taken together, suggest a role for vitamin D derivatives and retinoids on C 2 C 12 cells.