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Nuclear Factor of Activated T Cells (NFAT1‐C) Represses the Enhancer II and Pregenomic Promoter (EnII/Cp) of Hepatitis B Virus (HBV) Through its Responsive Site GGAGA and Nullifies the HBx‐Driven Transcriptional Activation
Author(s) -
Lee Joong Hyuk,
Rho Hyune Mo
Publication year - 2001
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/152165401753311807
Subject(s) - hbx , enhancer , microbiology and biotechnology , hepatitis b virus , heterologous , chemistry , promoter , transcriptional regulation , biology , gene , virology , transcription factor , virus , gene expression , biochemistry
The immunosuppressant cyclosporin A (CsA)‐sensitive nuclear factor of activated T cells 1 (NFAT1) has been known to be a transcriptional regulator of cytokine and viral genes during the immune response. By analyses of serial deletion, mutation, and heterologous promoter assay, we report here that the CsA‐sensitive NFAT1‐C represses the transcriptional activity of enhancer II and pregenomic promoter (EnII/Cp) of HBV through the NFAT1‐C responsive site (GGAGA, nt 1603‐1618) and nullifies the HBx‐driven transcriptional activation of the EnII/Cp of HBV in a dose‐dependent manner. These results suggest that a CsA‐sensitive NFAT1‐C may control the viral activity in HBV‐infected cells by inhibiting the EnII/Cp and nullifying the HBx‐driven transcriptional activation.