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Identification of a Transactivation Motif in the CLN3 Protein
Author(s) -
Leung K. Y.,
Greene N. D. E.,
Munroe P. B.,
Mole S. E.
Publication year - 2001
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/152165401317190798
Subject(s) - transactivation , repressor lexa , transcription factor , transcription (linguistics) , dna binding domain , biology , dna binding protein , dna , activator (genetics) , microbiology and biotechnology , genetics , chemistry , repressor , gene , linguistics , philosophy
A transactivation motif has been identified in the neurodegenerative disease protein, CLN3. The C‐terminal domain (residues 394‐438) of CLN3 can function as a transcriptional activator when fused to the DNA binding domain, LexA. A series of deletion and substitution constructs have been generated to identify the essential region for transactivation. A similar motif is also present in the POU domain transcription factor, nubbin. However, this domain alone does not activate transcription, allowing further localisation of the critical residues in CLN3 required for activity.