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Disaccharide Modulation of the Mitochondrial Membrane Fluidity Changes Induced by the Membrane Potential
Author(s) -
Ricchelli Fernanda,
Camerin Monica,
Beghetto Claudia,
Crisma Marco,
Moretto Vittorio,
Gobbo Silvano,
Salvato Benedetto,
Salet Christian,
Moreno Giuliana
Publication year - 2001
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216540117492
Subject(s) - membrane fluidity , membrane , chemistry , biophysics , disaccharide , fluorescence anisotropy , inner mitochondrial membrane , depolarization , membrane structure , membrane potential , membrane protein , membrane lipids , monosaccharide , phospholipid , biochemistry , biology
The influence of the medium composition on the dynamic properties of mitochondrial membranes on depolarization was studied by following the fluorescence anisotropy changes of mitochondriabound 1,6‐diphenyl‐1,3,5‐hexatriene (DPH) and hematoporphyrin (HP) as reporters, respectively, of lipid and protein regions. On collapse of the potential, the membrane fluidity increased in NaCl‐, KCl‐, and monosaccharide‐based media and decreased in disaccharides. Infrared spectroscopy experiments suggested that disaccharides likely change water's structure and association on the membrane surface. These results indicate that disaccharides induce membrane perturbation, which may interfere in the study of structure‐function correlation in biological membranes.