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In Vivo ESR Study on Hepatic Reduction of a Nitroxide Radical after Administration of Glucose in Rats
Author(s) -
Tada Mika,
Yokoyama Hidekatsu,
Toyoda Yoshiko,
Ohya Hiroaki,
Ogata Tateaki,
Kamada Hitoshi
Publication year - 2001
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216540117208
Subject(s) - nitroxide mediated radical polymerization , in vivo , chemistry , reduction (mathematics) , pharmacology , medicine , biology , organic chemistry , radical polymerization , microbiology and biotechnology , copolymer , polymer , geometry , mathematics
The in vivo reducing ability of a nitroxide radical, 4‐hydroxyl‐2,2,6,6‐tetramethylpiperidin‐1‐oxyl (TEMPOL), in the liver of rats was estimated by using an electron spin resonance spectrometer equipped with a surface‐coil‐type resonator following administration of glucose. Both oral and intravenous administration of glucose significantly enhanced the reduction capacity. In vitro studies show that the reduction site of TEMPOL in the hepatic homogenate is located in the mitochondrial respiratory chain and microsomal electron transport system. These findings indicate that the enhancement of activity of these systems caused by the glucose administration prompts reduction of TEMPOL in the liver.