The Presence of a Glycosyl Phosphatidylinositol‐Anchored α‐Mannosidase in Boar Sperm | Zendy

Kuno Masako | Zendy; Yonezawa Naoto | Zendy; Amari Shinji | Zendy; Hayashi Masayuki | Zendy; Ono Yuichiro | Zendy; Kiss Laszlo | Zendy; Sonohara Kuniharu | Zendy; Nakano Minoru | Zendy

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The Presence of a Glycosyl Phosphatidylinositol‐Anchored α‐Mannosidase in Boar Sperm

Author(s) -

Kuno Masako,

Yonezawa Naoto,

Amari Shinji,

Hayashi Masayuki,

Ono Yuichiro,

Kiss Laszlo,

Sonohara Kuniharu,

Nakano Minoru

Publication year - 2000

Publication title -

iubmb life

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.132

H-Index - 113

eISSN - 1521-6551

pISSN - 1521-6543

DOI - 10.1080/15216540050167016

Subject(s) - chemistry , kiss (tnc) , library science , computer science , computer network

alpha-Mannosidase and beta-galactosidase were released from boar sperm into the medium by treatment with calcium ionophore A23187 or by 0.2% Brij-35/2% acetic acid. About half as much alpha-mannosidase activity as that in the acid extract was recovered by digestion with phosphatidylinositol-specific phospholipase C (PI-PLC), whereas the liberation rate of beta-galactosidase treated with PI-PLC was low. These results suggest that some alpha-mannosidase is anchored in the plasma membrane of the acrosomal region by attachment to the lipid phosphatidylinositol and that beta-galactosidase is localized mainly in the acrosome or integrated in the plasma membrane by a spanning stretch of hydrophobic peptides. beta-Galactosidase, which is present as an oligomers in the acid extract of sperm, dissociated into monomers under weakly alkaline conditions; under acidic conditions, the monomers associated again. No pH-sensitive association-dissociation of alpha-mannosidase was observed.

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