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Studies on Insulin/IGF‐1 Hybrid and IGF‐1 Growth‐Promoting Functional Region
Author(s) -
Wang Ping,
Cai RuoRong,
Feng YouMin,
Zhang YouShang
Publication year - 2000
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216540050033203
Subject(s) - insulin , insulin like growth factor , domain (mathematical analysis) , chemistry , microbiology and biotechnology , growth factor , medicine , endocrinology , biology , biochemistry , receptor , mathematics , mathematical analysis
Single‐chain insulin/IGF‐1 hybrid‐[Ins/IGF‐1(C)], single‐chain porcine insulin precursor‐(PIP), and B10Asp PIP were prepared by protein engineering. Their growth‐promoting activities in mouse breast cancer cell line GR2H6 are 10, 0.2, and 2 times that of insulin, respectively, and 29%, 0.6%, and 6% of that of IGF‐1, indicating that the C domain and 9Glu of IGF‐1 are important for its growth‐promoting activity. Given these results and previous reports, we propose that the C domain, 9Glu, and 23Phe‐26Asn beta bend are involved in the growth‐promoting functional region of IGF‐1.