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Two Systems for Phosphate Uptake in Yarrowia lipolytica Cells Grown at Acidic Conditions
Author(s) -
Zvyagilskaya Renata,
Allard Peter,
Persson Bengt L.
Publication year - 2000
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216540050022476
Subject(s) - yarrowia , derepression , pi , phosphate , extracellular , chemistry , biochemistry , intracellular , cotransporter , yeast , intracellular ph , inorganic phosphate , organic chemistry , gene expression , psychological repression , gene , sodium
Inorganic phosphate (Pi) is accumulated by Yarrowia lipolytica cells grown at acidic pH conditions by two kinetically discrete H+/Pi‐ cotransport systems with apparent Km values for Pi of 12‐18 muM and 2‐3 mM Pi at pH 5.5, respectively. One of these is derepressible and operates at low external Pi concentrations; the other is most likely constitutively expressed and comes into play at high Pi concentrations. The derepression of the high‐affinity Pi transport system is under the control of available extracellular Pi as well as the amount of intracellular polyphosphates stores. Characteristics of the Pi transport behavior in Yarrowia lipolytica are discussed.