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Failure of 5‐HT 3 receptors in regulation of ethanol‐induced ascorbic acid release in rat striatum
Author(s) -
Wu ChunFu,
Liu Jing,
Liu Wen,
Consolo Silvana,
Huang Mei,
Yang JingYu
Publication year - 2001
Publication title -
addiction biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.445
H-Index - 78
eISSN - 1369-1600
pISSN - 1355-6215
DOI - 10.1080/13556210020020157
Subject(s) - ascorbic acid , microdialysis , chemistry , agonist , serotonin , antagonist , pharmacology , striatum , medicine , endocrinology , ethanol , 5 ht receptor , receptor antagonist , receptor , biochemistry , extracellular , dopamine , biology , food science
Previous studies have shown that the serotonergic system was involved in ethanol‐induced striatal ascorbic acid release in rats. In the present study the possible role of 5‐HT 3 receptors in ethanol‐induced striatal ascorbic acid release was investigated in rats using 5‐HT 3 antagonists ondansetron, DAU 6215 and 5‐HT 3 agonist 2‐methyl‐serotonin. Extracellular level of ascorbic acid in the striatum was determined by means of in vivo microdialysis coupled to HPLC with electrochemical detection. Ethanol (3 g/kg, i.p.) induced a significant increase in ascorbic acid release. Ondansetron (0.2 and 2.0 mg/kg, i.p.), DAU 6215 (0.06, 0.12 and 0.24 mg/kg, i.p.) and 2‐methyl‐serotonin (250 μg/rat, i.c.v.), administered 10 minutes before 0.15 M NaCl or ethanol (3 g/kg, i.p.), affect neither the basal nor the ethanol‐induced ascorbic acid release in rat striatum. 2‐Methylserotonin, at a dose of 500 μg/rat, i.c.v., increased the basal, but did not affect the ethanol‐induced ascorbic acid release in rat striatum. However, ritanserin (1 mg/kg, s.c.), a 5‐HT 2 receptor antagonist, and BIMU 8 (40 μg/rat, i.c.v.), a 5‐HT 4 agonist, significantly antagonized ethanol‐induced ascorbic acid release. These results suggest that 5‐HT 3 receptors, which form a part of cation channels, may not be involved in ethanolinduced striatal ascorbic acid release.