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Host Immune Competence and Local Ischemia Affects the Functionality of Engineered Vasculature
Author(s) -
CHEN RUTH R.,
SNOW JONATHAN K.,
PALMER JACK P.,
LIN ANGELA S.,
DUVALL CRAIG L.,
GULDBERG ROBERT E.,
MOONEY DAVID J.
Publication year - 2007
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.1080/10739680601131101
Subject(s) - perfusion , ischemia , immune system , angiogenesis , vascular endothelial growth factor , medicine , in vivo , immunology , vegf receptors , biology , microbiology and biotechnology
Objective: Localized and sustained delivery of vascular endothelial growth factor (VEGF) is a promising approach to overcome the limited efficacy of bolus delivery. The authors examined the effects of host immune competence and local ischemia on the functionality of new vessel networks formed with this approach. Methods: Vessel structure and perfusion resulting from implantation of porous 85:15 poly(lactide‐co‐glycolide) scaffolds releasing VEGF 165 were measured in both subcutaneous tissue and ischemic hindlimbs of immune competent C57BL/6 and immune deficient SCID mice. Results: Sustained VEGF delivery resulted in a similar ∼100% increase in vessel density within scaffolds in both implant sites, and both animal models. However, the resulting perfusion within scaffolds implanted in subcutaneous tissue increased modestly versus control (18–35%), while perfusion increased 52–110% above control when VEGF‐releasing scaffolds were placed in ischemic hindlimbs of C57BL/6 or SCID mice. VEGF delivery improved perfusion in the entire ischemic limb (55 ± 18% of the normal value by week 6; 138% increase over control) in SCID mice. Although C57BL/6 mice demonstrated spontaneous recovery from ischemia, VEGF delivery accelerated recovery as compared to control. Conclusions: Localized and sustained VEGF delivery can create functional vasculature that amplifies recovery of tissue ischemia. However, increases in local and regional perfusion were highly dependent on the implantation site and the animal model.