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In Vivo Phosphorescence Imaging of p O 2 Using Planar Oxygen Sensors
Author(s) -
BABILAS PHILIPP,
LIEBSCH GREGOR,
SCHACHT VIVIEN,
KLIMANT INGO,
WOLFBEIS OTTO S.,
SZEIMIES ROLFMARKUS,
ABELS CHRISTOPH
Publication year - 2005
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.1080/10739680591003314
Subject(s) - in vivo , planar , porphyrin , chemistry , materials science , analytical chemistry (journal) , platinum , oxygen sensor , electrode , biomedical engineering , oxygen , photochemistry , chromatography , medicine , biochemistry , computer graphics (images) , microbiology and biotechnology , organic chemistry , computer science , biology , catalysis
Objective: Oxygen‐dependent quenching of luminescence of metal porphyrin complexes has been used to image the p O 2 distribution over tumor and normal tissue. Methods: An experimental setup is described using a platinum(II)–octaethyl–porphyrin immobilized in a polystyrene matrix as transparent planar sensor. Results: Sensitivity over a broad range is high at low p O 2 values (± 0.2 mm Hg at 0 mm Hg; ± 1.5 mm Hg at 160 mm Hg p O 2 ). Due to intrinsically referencing via lifetime encoding there was no modification of the sensor response in vivo in the dorsal skinfold chamber model with amelanotic melanoma (A‐MEL‐3) in awake hamsters when compared to the in vitro calibration. p O 2 measurements over normal tissue (25.8 ± 5.1 mm Hg) and tumor tissue (9.2 ± 5.1 mm Hg) were in excellent agreement with previous results obtained in this model using a surface multiwire electrode. Conclusions: Using the presented method the surface p O 2 distribution can be mapped with a high temporal resolution of approximately 100 ms and a spatial resolution of at least 25 μ m. Moreover, the transparent sensor allows the simultaneous visualization of the underlying microvasculature.