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Telomerase‐Immortalized Lymphatic and Blood Vessel Endothelial Cells are Functionally Stable and Retain Their Lineage Specificity
Author(s) -
SCHOPPMANN SEBASTIAN F.,
SOLEIMAN AFSCHIN,
KALT ROMANA,
OKUBO YUKARI,
BENISCH CHRISTIAN,
NAGAVARAPU USHA,
HERRON G. SCOTT,
GELEFF SILVANA
Publication year - 2004
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.1080/10739680490425967
Subject(s) - lymphatic endothelium , lymphatic system , biology , endothelial stem cell , flow cytometry , immortalised cell line , endothelium , cell culture , microbiology and biotechnology , telomerase , telomerase reverse transcriptase , immunofluorescence , cell type , immunology , cell , pathology , in vitro , gene , antibody , genetics , medicine
Objective: Microvasculature plays an important role in a variety of physiological and pathological processes. The authors have previously shown that primary cultures of human microvascular endothelial cells consist of 2 distinct populations of blood vascular and lymphatic endothelial cells. These subtypes are ephemeral and lose purity through passaging. To generate reproducible in vitro and in vivo experiments stable blood and lymphatic endothelial cell lines are an essential prerequisite. Methods: In this study they have used human telomerase gene‐immortalized nontransformed human microvascular endothelial cell cloned pure cultures of blood and lymphatic endothelial cell subpopulations. Flow cytometry, immunofluorescence, Northern and Western blotting, microarray gene analysis, as well as basic functional assays were used to characterize these clones. Results and Conclusions: Immortalized blood and lymphatic subpopulations are stable and functionally specialized cell lineages that expressed pan‐endothelial and cell‐type‐specific markers. They are excellent candidates for long‐term culture studies on microvascular‐related diseases.