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Expression of Cytochrome P450‐4A Isoforms in the Rat Cremaster Muscle Microcirculation
Author(s) -
WANG JINGLI,
MAIER KRISTOPHER G.,
ROMAN RICHARD J.,
DE LA CRUZ LOURDES,
ZHU JIAXUAN,
HENDERSON LISA,
LOMBARD JULIAN H.
Publication year - 2004
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.1080/10739680490266225
Subject(s) - cremaster muscle , microcirculation , gene isoform , cytochrome p450 , chemistry , microbiology and biotechnology , anatomy , medicine , endocrinology , pharmacology , biology , biochemistry , enzyme , gene
Objective: This study sought to identify any specific cytochrome P450 (CYP450) ‐4A enzyme isoforms expressed in arterioles and/or the surrounding parenchymal tissue of the rat cremaster muscle. Methods: RT‐PCR was used to detect the presence of specific CYP450‐4A isoforms in isolated muscle fibers and arterioles from the cremaster muscle of Sprague‐Dawley rats; CYP450‐4A protein expression was determined by Western blotting. Results: CYP450‐4A3 mRNA was expressed in isolated muscle fibers and in cremasteric arterioles, while CYP450‐4A8 mRNA was expressed only in cremasteric arterioles. CYP450‐4A1 and CYP450‐4A2 mRNA were not expressed in arterioles and skeletal muscle cells, although all four isoforms were strongly expressed in the liver. CYP450‐4A protein was detected in both the isolated muscle fibers and in the isolated arterioles. Conclusions: The present study identifies the specific pattern of cytochrome P450‐4A isoform expression in arterioles and parenchymal cells of the skeletal muscle microcirculation, and supports the hypothesis that the cytochrome P‐450 enzymes may play a role in the regulation of microvascular function in the skeletal muscle microcirculation.