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Inhibition of Grass Carp Reovirus Infection by DNA Aptamers against S10 Protein
Author(s) -
Liang Hongru,
Fu Xiaozhe,
Liu Lihui,
Lin Qiang,
Guo Huizhi,
Li Yonggang,
Liu JiaXu,
Huang Zhibin,
Li Ningqiu
Publication year - 2017
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1080/08997659.2017.1293572
Subject(s) - aptamer , grass carp , biology , virology , dna , gene , systematic evolution of ligands by exponential enrichment , outbreak , carp , microbiology and biotechnology , fish <actinopterygii> , genetics , fishery , rna
Grass Carp reovirus (GCRV) is one of the most pathogenic agents among aquareovirus isolates and has the ability to cause a severe epidemic outbreak of hemorrhagic disease, thus resulting in both a high mortality rate during the culture of Grass Carp Ctenopharyngodon idella and an enormous economic loss. Aptamers have been demonstrated to have strong promising applications in antiviral drug development. In the present study, a complementary DNA fragment encoding the S10 gene of GCRV was cloned. The S10 protein was expressed and purified. Aptamers for S10 protein were selected by the method of selective evolution of ligands by exponential enrichment (SELEX), and their characteristics and antiviral actions were examined. All targeting‐selected aptamers formed a similar structure, forming a 5–7 base loop at the terminus. The results show that the aptamers could inhibit the GCRV infection. The most significant inhibitory effect was obsereved when the aptamers were added to the cell culture for 1 h before the cells were infected by GCRV. Our data showed that these novel molecular agents could be considered suitable candidates for anti‐GCRV therapy. Received August 23, 2016; accepted February 5, 2017Published online April 5, 2017