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Assessment of Detoxifying Markers for Florfenicol in Rainbow Trout Liver
Author(s) -
Elia Antonia Concetta,
Pacini Nicole,
Fioravanti Maria Letizia,
Dörr Ambrosius Josef Martin,
Zaccaroni Annalisa,
Parmeggiani Alba Maria,
Gustinelli Andrea,
Mordenti Oliviero,
Abete Maria Cesarina,
Prearo Marino
Publication year - 2016
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1080/08997659.2016.1206637
Subject(s) - florfenicol , rainbow trout , biology , fish <actinopterygii> , zoology , fishery , microbiology and biotechnology , antibiotics
Florfenicol (FF) is employed in fish farms to contest or prevent bacterial infections. However, this pharmaceutical may produce reactive oxygen species that may cause biochemical changes in antibiotic‐treated fish. The aim of this study was to evaluate the effect of FF on Rainbow Trout Oncorhynchus mykiss treated for 10 d with 7.5 and 15 mg/kg FF followed by a withdrawal period of 5 d. Superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione S‐transferase, glyoxalase I and glyoxalase II, total glutathione, lactic dehydrogenase, and alkaline phosphatase were investigated in the livers of treated and untreated fish. A general impairment of antioxidant enzymes and metabolic indicators was measured in FF‐treated Rainbow Trout. Onset of oxidative damage may have occurred during the antibiotic treatment as a consequence of the effect of FF toxicity at mainly the highest dose. Nevertheless, the rise in levels of total glutathione and glutathione S‐transferase even after the withdrawal period may shield the antibiotic‐mediated oxidative processes. Received December 22, 2015; accepted May 26, 2016 Published online October 28, 2016