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Analysis of Short‐Term Cortisol Stress Response in Channel Catfish by Anesthetization with Metomidate Hydrochloride and Tricaine Methanesulfonate
Author(s) -
Smith M. S.,
Booth N. J.,
Peterson B. C.,
Stephens W. S.,
Goudie C. A.,
Simco B. A.
Publication year - 2015
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1080/08997659.2015.1047537
Subject(s) - ictalurus , catfish , sedation , anesthetic , fish <actinopterygii> , anesthesia , hydrochloride , biology , chemistry , endocrinology , medicine , fishery , biochemistry
The ability of the anesthetics metomidate hydrochloride and tricaine methanesulfonate (MS‐222) to mitigate the cortisol stress response of Channel Catfish Ictalurus punctatus was evaluated during a 10‐min confinement stress. The cortisol concentrations of Channel Catfish anesthetized in metomidate hydrochloride remained consistent throughout the 10‐min exposure; however, for fish anesthetized with MS‐222 and nonanesthetized fish, cortisol concentrations were approximately 7‐ and 22‐fold higher, respectively, than the baseline concentrations. While both anesthetics reduced cortisol concentrations relative to those of nonanesthetized fish, these results suggest that MS‐222 is an appropriate anesthetic to use during the initial 5 min of sedation and that metomidate hydrochloride is appropriate for longer periods of sedation. Received November 18, 2014; accepted April 9, 2015