Premium
Relationship between Permeability Glycoprotein (P‐gp) Gene Expression and Enrofloxacin Metabolism in Nile Tilapia
Author(s) -
Hu Kun,
Cheng Gang,
Zhang Haixin,
Wang Huicong,
Ruan Jiming,
Chen Li,
Fang Wenhong,
Yang Xianle
Publication year - 2014
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1080/08997659.2013.860059
Subject(s) - nile tilapia , gene expression , biology , p glycoprotein , pharmacology , metabolism , drug metabolism , oreochromis , kidney , gene , first pass effect , oral administration , drug , endocrinology , biochemistry , fish <actinopterygii> , antibiotics , fishery , multiple drug resistance
The aim of this study was to analyze the influence of permeability glycoprotein (P‐gp) gene expression on enrofloxacin (ENR) metabolism in aquatic animals. Nile Tilapia Oreochomis niloticus were fed different doses of ENR ranging from 0 to 80 mg/kg. The P‐gp gene expression levels were determined by quantitative real‐time PCR (qRT‐PCR) at indicated time points after drug administration. Drug metabolism was determined by HPLC. The P‐gp gene expression in liver and kidney was greatly enhanced 30 min after ENR administration at 40 mg/kg, peaked 3 h after drug administration, and then gradually decreased. Thirty minutes after a single oral administration of ENR (0, 20, 40, or 80 mg/kg), the P‐gp gene expression increased in a dose‐dependent manner. The P‐gp gene expression levels in the kidney were significantly higher than those in the liver. Additionally, the metabolic rate of ENR in kidney was more rapid than that in liver. Furthermore, a close correlation was found between P‐gp gene expression and ENR concentrations. These results suggest that P‐gp may be involved in the ENR metabolism process in Nile Tilapia, providing a novel model for the potential utility of gene expression and drug metabolism studies in aquatic animals. Received July 9, 2013; accepted October 17, 2013