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The Endemic Copepod Calanus pacificus californicus as a Potential Vector of White Spot Syndrome Virus
Author(s) -
MendozaCano Fernando,
SánchezPaz Arturo,
TeránDíaz Berenice,
GalvánAlvarez Diego,
EncinasGarcía Trinidad,
EnríquezEspinoza Tania,
HernándezLópez Jorge
Publication year - 2014
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1080/08997659.2013.852635
Subject(s) - copepod , biology , white spot syndrome , biological dispersal , shrimp , zoology , crustacean , virus , outbreak , vector (molecular biology) , ecology , virology , fishery , genetics , gene , population , demography , sociology , recombinant dna
The susceptibility of the endemic copepod Calanus pacificus californicus to white spot syndrome virus (WSSV) was established by the temporal analysis of WSSV VP28 transcripts by quantitative real‐time PCR (qRT‐PCR). The copepods were collected from a shrimp pond located in Bahia de Kino Sonora, Mexico, and challenged per os with WSSV by a virus–phytoplankton adhesion route. Samples were collected at 0, 24, 48 and 84 h postinoculation (hpi). The VP28 transcripts were not detected at early stages (0 and 24 hpi); however, some transcript accumulation was observed at 48 hpi and gradually increased until 84 hpi. Thus, these results clearly show that the copepod C. pacificus californicus is susceptible to WSSV infection and that it may be a potential vector for the dispersal of WSSV. However, further studies are still needed to correlate the epidemiological outbreaks of WSSV with the presence of copepods in shrimp ponds. Received May 22, 2013; accepted October 2, 2013.