Open AccessDetermination of blood sirolimus concentrations in liver and kidney transplant recipients using the Innofluor® fluorescence polarization immunoassay: Comparison with the microparticle enzyme immunoassay and high-performance liquid chromatography-ultraviolet method
Author(s) -
L.F. Bouzas,
Jesús Hermida,
J. Carlos Tutor
Publication year - 2009
Publication title -
upsala journal of medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.808
H-Index - 41
eISSN - 2000-1967
pISSN - 0300-9734
DOI - 10.1080/03009730802608254
Subject(s) - medicine , immunoassay , sirolimus , fluorescence polarization immunoassay , chromatography , enzyme , liver enzyme , microparticle , immunology , biochemistry , antibody , optics , chemistry , physics
Although high-performance liquid chromatography (HPLC) is the method of choice for blood sirolimus determination, the microparticle enzyme immunoassay (MEIA) run on the IMx analyser is widely used in therapeutic monitoring of this immunosuppressant agent. The aim of our study was to evaluate the possible determination of sirolimus using the fluorescence polarization immunoassay (FPIA) commercialized for everolimus quantification.