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Candidate Genetic Markers Associated with Anadromy in Oncorhynchus mykiss of the Klickitat River
Author(s) -
Narum Shawn R.,
Zendt Joseph S.,
Frederiksen Chris,
Campbell Nate,
Matala Andrew,
Sharp William R.
Publication year - 2011
Publication title -
transactions of the american fisheries society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.696
H-Index - 86
eISSN - 1548-8659
pISSN - 0002-8487
DOI - 10.1080/00028487.2011.588131
Subject(s) - biology , single nucleotide polymorphism , rainbow trout , candidate gene , genetics , snp , genetic marker , allele , logistic regression , genetic association , population , ancestry informative marker , univariate , genotype , evolutionary biology , multivariate statistics , fish <actinopterygii> , gene , fishery , medicine , statistics , environmental health , mathematics
Both environmental and genetic factors influence anadromy in rainbow trout Oncorhynchus mykiss , but the genetic mechanisms that contribute to migratory selection are not well understood. In this study, we used a limited genome scan approach to identify candidate genetic markers associated with anadromy in 10 populations of O. mykiss from the Klickitat River, Washington. From an initial panel of 96 single‐nucleotide polymorphism (SNP) markers, we identified 3 SNPs that were significantly associated with anadromy after accounting for underlying population structure and selective environmental conditions. Univariate logistic regression of allele frequencies and residency/anadromy were also significant, and thus three SNPs were considered candidate markers associated with anadromy ( Omy_IL6–320, Omy_LDHB‐2_i6 , and Omy_ndk‐152 ). A multivariate logistic model was developed from the allele frequencies of these three markers to predict the potential for anadromy in natural populations. This model was applied to eight additional populations of O. mykiss to evaluate its utility. The results of this study indicate that these markers are strong candidates for association with anadromy in O. mykiss in the Klickitat River, but further testing is needed to evaluate this association across more of this species’ range. Common‐garden experiments may also help clarify the association of these candidate markers with the smoltification phenotypes of individual fish.