Open AccessExpression of human Biot2 and its potential function on carcinogenesis in endometrial cancer
Author(s) -
YANGMEI SHEN,
YANGMEI SHEN,
XIANG HE,
HONGXIN DENG,
HANSHUO YANG,
FENG PENG,
YUPING XIE,
YUQUAN WEI,
XIA ZHAO
Publication year - 2007
Publication title -
acta obstetricia et gynecologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.401
H-Index - 102
eISSN - 1600-0412
pISSN - 0001-6349
DOI - 10.1080/00016340701736946
Subject(s) - endometrial cancer , endometrium , carcinogenesis , grading (engineering) , medicine , cancer , menstrual cycle , pathology , biology , hormone , ecology
Background . Rat Biot2 is a novel gene first reported and named by our laboratory. We wanted to study the expression profile and reveal the function of its human homologous gene (human Biot2, h Biot2) on carcinogenesis in endometrial cancer. Methods . Using RT‐PCR, we tested h Biot2 expression profile in normal human tissues from infants and adults. Using real‐time RT‐PCR, we tested different expression quantities of h Biot2 between an endometrial cancer group and a normal endometrium group, between different histological grading groups in endometrial cancer, and between different stage groups during the menstrual cycle. RNA‐RNA ISH tested the expression location of h Biot2 in normal and cancer tissues. Results . Three kinds of novel shearings of h Biot2 in different normal tissues were obtained. Normal organs expressing h Biot2 in infants, did not express h Biot2 in adults. h Biot2 expression was higher in endometrial cancer tissue compared to normal endometrium tissue. h Biot2 expression was higher in the Grade 2–3 group with endometrial cancer compared to the Grade 1 group, and it was higher in the proliferative phase than in the secretory phase of a normal endometrium. h Biot2 was expressed mainly in the parenchymal cells. Conclusions . Three shearings of h Biot2 may suggest that it has different functions in different organs. The expression difference of h Biot2 between infants and adults, cancerous and normal tissues, different histological gradings in endometrial cancer, different stages during the menstrual cycle in the normal endometrium suggests that h Biot2 may have the potential to induce carcinogenesis in endometrial cancer.